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Immunogenicity <t>of</t> <t>MVA-BN-loaded</t> DMN patches in mice. ( a ) Log 10 -transformed endpoint titers of MPXV-specific total IgG in mouse serum at 14 and 28 days post-boost immunization, detected by a commercial combined ELISA kit pre-coated with four MPXV antigens in a single well. ( b ) PRNT 50 endpoint titers of MPXV neutralizing antibodies in mouse serum at 7 days post-boost immunization. The dashed line in both panels indicates the mean value of the PBS blank control group, defined as the lower detection limit of the corresponding assay. Data are presented as mean ± SD (n = 5); ns, non-significant ( p > 0.05).
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(a) Tyndall Effect (green laser) for <t>standard</t> <t>h-BN</t> powder, pulsed, and non-pulsed <t>h-BN</t> <t>NPs,</t> (b) XRD peaks of h-BN nanoparticles and standard h-BN, and (c) Raman spectroscopy of standard h-BN particles and h-BN NPs, (d) Reflectance measurement of prepared devices.
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Immunogenicity of MVA-BN-loaded DMN patches in mice. ( a ) Log 10 -transformed endpoint titers of MPXV-specific total IgG in mouse serum at 14 and 28 days post-boost immunization, detected by a commercial combined ELISA kit pre-coated with four MPXV antigens in a single well. ( b ) PRNT 50 endpoint titers of MPXV neutralizing antibodies in mouse serum at 7 days post-boost immunization. The dashed line in both panels indicates the mean value of the PBS blank control group, defined as the lower detection limit of the corresponding assay. Data are presented as mean ± SD (n = 5); ns, non-significant ( p > 0.05).

Journal: Vaccines

Article Title: Dissolvable Microneedle Delivery of a Replication-Deficient Orthopoxvirus Vaccine: Formulation Screening and Immunogenicity Evaluation for Monkeypox Prevention

doi: 10.3390/vaccines14030276

Figure Lengend Snippet: Immunogenicity of MVA-BN-loaded DMN patches in mice. ( a ) Log 10 -transformed endpoint titers of MPXV-specific total IgG in mouse serum at 14 and 28 days post-boost immunization, detected by a commercial combined ELISA kit pre-coated with four MPXV antigens in a single well. ( b ) PRNT 50 endpoint titers of MPXV neutralizing antibodies in mouse serum at 7 days post-boost immunization. The dashed line in both panels indicates the mean value of the PBS blank control group, defined as the lower detection limit of the corresponding assay. Data are presented as mean ± SD (n = 5); ns, non-significant ( p > 0.05).

Article Snippet: Consequently, the World Health Organization (WHO) recommends three smallpox vaccines—LC16m8 (KM Biologics), MVA-BN (Bavarian Nordic; a replication-deficient attenuated live vaccine), and ACAM2000 (Emergent BioSolutions; a replication-competent attenuated live vaccine)—for individuals at risk of MPXV infection [ ].

Techniques: Immunopeptidomics, Transformation Assay, Enzyme-linked Immunosorbent Assay, Control

(a) Tyndall Effect (green laser) for standard h-BN powder, pulsed, and non-pulsed h-BN NPs, (b) XRD peaks of h-BN nanoparticles and standard h-BN, and (c) Raman spectroscopy of standard h-BN particles and h-BN NPs, (d) Reflectance measurement of prepared devices.

Journal: ACS Omega

Article Title: Optoelectronic Enhancement in Nanostructured h‑BN Synthesized Using Pulsed Ultrasonication

doi: 10.1021/acsomega.5c12635

Figure Lengend Snippet: (a) Tyndall Effect (green laser) for standard h-BN powder, pulsed, and non-pulsed h-BN NPs, (b) XRD peaks of h-BN nanoparticles and standard h-BN, and (c) Raman spectroscopy of standard h-BN particles and h-BN NPs, (d) Reflectance measurement of prepared devices.

Article Snippet: The surface texture, topography, and size distribution of h-BN NPs were investigated by using a JEOL JSM-7900F advanced analytical field emission scanning electron microscope (FESEM) at different electron-accelerating potentials ranging from 5 to 15 kV.

Techniques: Raman Spectroscopy

(a) SEM of commercial h-BN powder. (b) Size distribution of pulsed h-BN NPs with ImageJ size distribution inset. (c) SEM of non-pulsed h-BN NPs with ImageJ size distribution inset. (d) DLS size distributions comparing commercial, non-pulsed, and pulsed h-BN samples.

Journal: ACS Omega

Article Title: Optoelectronic Enhancement in Nanostructured h‑BN Synthesized Using Pulsed Ultrasonication

doi: 10.1021/acsomega.5c12635

Figure Lengend Snippet: (a) SEM of commercial h-BN powder. (b) Size distribution of pulsed h-BN NPs with ImageJ size distribution inset. (c) SEM of non-pulsed h-BN NPs with ImageJ size distribution inset. (d) DLS size distributions comparing commercial, non-pulsed, and pulsed h-BN samples.

Article Snippet: The surface texture, topography, and size distribution of h-BN NPs were investigated by using a JEOL JSM-7900F advanced analytical field emission scanning electron microscope (FESEM) at different electron-accelerating potentials ranging from 5 to 15 kV.

Techniques:

(a) Normalized UV enhancement ratio vs time graph for the commercial (uncoated) LDR, standard h-BN-coated LDR, pulsed h-BN NP-coated LDR, and non-pulsed h-BN NPs-coated LDR under 365 nm illumination for multiple cycles. (b) UV enhancement ratio vs time graph for a single cycle; the inset image contains a zoomed-out section of the graph when the UV light is turned ON.

Journal: ACS Omega

Article Title: Optoelectronic Enhancement in Nanostructured h‑BN Synthesized Using Pulsed Ultrasonication

doi: 10.1021/acsomega.5c12635

Figure Lengend Snippet: (a) Normalized UV enhancement ratio vs time graph for the commercial (uncoated) LDR, standard h-BN-coated LDR, pulsed h-BN NP-coated LDR, and non-pulsed h-BN NPs-coated LDR under 365 nm illumination for multiple cycles. (b) UV enhancement ratio vs time graph for a single cycle; the inset image contains a zoomed-out section of the graph when the UV light is turned ON.

Article Snippet: The surface texture, topography, and size distribution of h-BN NPs were investigated by using a JEOL JSM-7900F advanced analytical field emission scanning electron microscope (FESEM) at different electron-accelerating potentials ranging from 5 to 15 kV.

Techniques: